The only parameters that should be necessary to change are in the top section with
DIRECTORY=/mnt/research/germs/Schuyler/Projects/X
RAWDAT_DIRECTORY=$DIRECTORY/original
MAPPING_FILE=$RAWDAT_DIRECTORY/map.txt
CORES=20
OVERLAP=10
MINLENGTH=210
MAXLENGTH=280
Q=10
CLUSTER=0.99
Feel free to explore other parameters within the code. As long as you have the raw data backed-up, there is no commands in this pipeline that should be able to make unfixable mistakes.
Schuyler Smith
Ph.D. Student - Bioinformatics and Computational Biology
Iowa State University. Ames, IA.